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Lucie Hradecka of Czech Republic lies down on the court due to injury during the singles' qualifying match against Monica Puig of Puerto Rico at 2017 WTA Wuhan Open in Wuhan cheap air max 2018 , capital of central China's Hubei Province, on Sept. 22, 2017. Lucie Hradecka retired due to injury. (XinhuaXiong Qi)

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SAN FRANCISCO cheap air max 2017 , Sept. 20 (Xinhua) -- Researchers in the United States have identified a key region within a protein that governs how accurately the gene-editing tool known as CRISPR-Cas9 homes in on a target deoxyribonucleic acid (DNA) sequence.

CRISPR is short for Clustered Regularly Interspaced Short Palindromic Repeats and Cas9 stands for CRISPR Associated Protein.

The researchers at the University of California, Berkeley, and Massachusetts General Hospital said they have tweaked the region within the Cas9 protein to produce a hyper-accurate gene editor with the lowest level of off-target cutting to date. Identified as a master controller of DNA cutting, the protein domain known as REC3 is a target for re-engineering to further improve accuracy and to minimize the chance that CRISPR-Cas9 will edit DNA at the wrong place.

Co-first author Janice Chen, a graduate student in the lab of Jennifer Doudna air max 1 master sale , the UC Berkeley professor of molecular and cell biology who was one of the first scientists to invent CRISPR-Cas9 technology, noted in a press release that "we have found that even minor alterations in the REC3 domain of Cas9 affect the differential between on- and off-target editing, which suggests that this domain is an obvious candidate for in-depth mutagenesis to improve targeting specificity."

Targeting specificity is a key consideration when doing gene therapy in humans.

Currently, scientists using CRISPR-Cas9 create a single-guide ribonucleic acid, or sgRNA air max 97 mens cheap , an RNA molecule that includes a chain of 20 ribonucleic acids that complements a specific 20-nucleic-acid DNA sequence they want to target, and attach it to Cas9. This guide RNA allows Cas9 to home in on the complementary DNA, bind to it and cut the double stranded helix.

However, the Cas9-sgRNA complex can bind to DNA that doesn't exactly match, leading to undesirable off-target cutting.

Doudna's lab discovered in 2015 a conformational switch of Cas9 that is activated when the RNA guide and DNA target match. They found that only when the RNA and DNA match closely does the 3D structure of Cas9 air max 97 womens size 7 , in particular the conformation of the HNH nuclease domain, change and activate the scissors of Cas9. But the process for sensing the nucleic acids upstream of the conformational switch remained unknown.

In the current study supported by the U.S. National Institutes of Health (NIH) and the U.S. National Science Foundation (NSF), Chen and her colleagues used a technique called single-molecule Forster resonance energy transfer (FRET) to precisely measure how the various protein domains in the Cas9-sgRNA protein complex - in particular REC3, REC2 and HNH - move when the complex binds to DNA.

The researchers uncovered that the REC3 domain is responsible for sensing the accuracy of target binding, which then signals the outward rotation of the REC2 domain to open a path for the HNH nuclease domain air max 97 all white womens , activating the scissors. They then showed that by mutating parts of REC3, it is possible to change the specificity of the Cas9 protein so that the HNH nuclease is not activated unless the guide RNA and target DNA match is very close.

They were able to engineer an improved hyper-accurate Cas9, dubbed HypaCas9, that retains its on-target efficiency but is slightly better at discriminating between on- and off-target sites in human cells.

"If you mutate certain amino acid residues in REC3, you can tweak the balance between Cas9 on-target activity and improve specificity; we were able to find the sweet spot where there is sufficient activity at the intended target but also a large reduction in off-target events air max 97 all black mens ," Chen was quoted as saying in the news release from UC Berkeley.

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Highlights of 10th China Acrobatics Golden Chrysanthemum Awards

Highlights of 10th China Acrobatics Golden Chrysanthemum Awards

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BEIJING, Nov.？22 (Xinhuanet) -- China's workforce is maturing and giving priority to different aspects of corporate culture, a new survey shows.

The 2013 MRIC Talent Report by recruitment firm MRICMRI China Group surveyed more than 5,000 Chinese professionals and managers on the Chinese mainland nike air max 97 ultra 17 rose gold , and in Hong Kong, Singapore and Taiwan.

It found that Chinese professionals were "increasingly being lured" by local corporations, as State-owned enterprises and domestic private-sector companies expand within the nation and abroad.

Chinese companies are "increasingly attractive when compared with Western (multinational corporations) in terms of career growth and development," wrote Christine Raynaud, chief executive officer of MRIC air max 97 silver bullet for sale , and Angie Eagan, managing director of MRIC in China.

Chinese companies may be able to "draw on strong nation. Cheap Old Skool White Cheap Old Skool Pink Cheap Old Skool Mens Cheap Old Skool Womens Cheap Old Skool Red Air Max 1 Sale Nike Air Max 97 Sale Cheap Nike Air Max 90 Cheap Air Max Shoes Cheap Air Max 97 Online